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for Recombinant FAK protein, activeContents
10 µg of Recombinant FAK protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol. Protein is supplied at 0.135 µg/µl.
for Recombinant FAK protein, activeApplication Notes
Recombinant FAK is suitable for kinase assays and Western blot. The molecular weight of the protein is ~145.394 kDa. The activity of the protein is ~ 1.5 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: PolyEY 0.5 µg/50 µl, Recombinant FAK: 200 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.
for Recombinant FAK protein, activeProtein Details
Recombinant human FAK protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids A2-H1052, accession number L13616 and N-terminally fused to GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.
for Recombinant FAK protein, activeGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
