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Recombinant FGR protein

Aliases: SRC2, c-fgr, c-src2, p55-Fgr, p58-Fgr, p55c-fgr, p58c-fgr
Catalog No: 31172 Format: 10 µg $390 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant FGR proteinContents

10 µg of Recombinant FGR protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant FGR proteinApplication Notes

Recombinant FGR is suitable for kinase assays and Western blot. The molecular weight of the protein is ~93.934 kDa. The activity of the protein is ~ 11 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: PolyEY 2 µg/50 µl, Recombinant FGR: 200 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.

for Recombinant FGR proteinProtein Details

Recombinant human FGR protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids M1-T529, accession number NM_005248.1 and N-terminally fused to a GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.

 
Recombinant FGR protein, active analyzed by SDS-PAGE gel.

Recombinant FGR protein tested by SDS-PAGE gel.
0.5 µg of FGR protein was analyzed using SDS/PAGE followed by Western blot with either an anti-FGR or anti-GST antibody.

for Recombinant FGR proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant FGR protein
 

Data Thumbnails

Recombinant FGR protein, active analyzed by SDS-PAGE gel.

SDS-PAGE gel of Recombinant FGR protein, active.
(Click image to enlarge and see details.)