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for Recombinant FGR protein, activeContents
10 µg of Recombinant FGR protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol. Protein is supplied at 0.111 µg/µl.
for Recombinant FGR protein, activeApplication Notes
Recombinant FGR is suitable for kinase assays and Western blot. The molecular weight of the protein is ~93.934 kDa. The activity of the protein is ~ 11 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: PolyEY 2 µg/50 µl, Recombinant FGR: 200 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.
for Recombinant FGR protein, activeProtein Details
Recombinant human FGR protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids M1-T529, accession number NM_005248.1 and N-terminally fused to GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.
Recombinant FGR protein tested by SDS-PAGE gel.
0.5 µg of FGR protein was analyzed using SDS/PAGE followed by Western blot with either an anti-FGR or anti-GST antibody.
for Recombinant FGR protein, activeGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
