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for Recombinant HCK protein, activeContents
10 µg of Recombinant HCK protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol. Protein is supplied at 0.143 µg/µl.
for Recombinant HCK protein, activeApplication Notes
Recombinant HCK is suitable for kinase assays and Western blot. The molecular weight of the protein is ~86.707 kDa. The activity of the protein is ~ 34 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: PolyEY 1 µg/50 µl, Recombinant HCK: 200 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.
for Recombinant HCK protein, activeProtein Details
Recombinant human HCK protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids M1-P505, accession number NM_002110 and N-terminally fused to GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.
Recombinant HCK protein tested by SDS-PAGE gel.
0.5 µg of HCK protein was analyzed using SDS/PAGE followed by Western blot with an anti-GST antibody.
for Recombinant HCK protein, activeGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
