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for Recombinant IGF1-R protein, activeContents
10 µg of Recombinant IGF1-R protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol. Protein is supplied at 0.09 µg/µl.
for Recombinant IGF1-R protein, activeApplication Notes
Recombinant IGF1-R is suitable for kinase assays and Western blot. The molecular weight of the protein is ~76.532 kDa. The activity of the protein is ~ 134 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: PolyEY 2 µg/50 µl, Recombinant IGF1-R: 20 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.
for Recombinant IGF1-R protein, activeProtein Details
Recombinant human IGF1-R protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids M954-C1367, accession number NM_000875 and N-terminally fused to GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.
Recombinant IGF1-R protein tested by SDS-PAGE gel.
0.5 µg of IGF1-R protein was analyzed using SDS/PAGE followed by Western blot with either an anti-IGF11-R or anti-GST antibody.
for Recombinant IGF1-R protein, activeGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
