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Recombinant ITK protein

Aliases: IL-2 Inducible T-Cell Kinase, Tyrosine-Protein Kinase LYK, EMT, LYK, LPFS1
Catalog No: 31180 Format: 10 µg $390 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant ITK proteinContents

10 µg of Recombinant ITK protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant ITK proteinApplication Notes

Recombinant ITK is suitable for kinase assays and Western blot. The molecular weight of the protein is ~101.727 kDa. The activity of the protein is ~ 11 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: PolyEY 1 µg/50 µl, Recombinant ITK : 200 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.

for Recombinant ITK proteinProtein Details

Recombinant human ITK protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids M1-L620, accession number NM_005546 and N-terminally fused to a GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.

 
Recombinant ITK protein, active analyzed by SDS-PAGE gel.

Recombinant ITK protein tested by SDS-PAGE gel.
0.5 µg of ITK protein was analyzed using SDS/PAGE followed by Western blot with an anti-GST antibody.

for Recombinant ITK proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant ITK protein
 

Data Thumbnails

Recombinant ITK protein, active analyzed by SDS-PAGE gel.

SDS-PAGE gel of Recombinant ITK protein, active.
(Click image to enlarge and see details.)