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Recombinant Proteins & Enzymes

Recombinant JAK2 protein

Aliases: Fd17
Catalog No: 31181 Format: 10 µg $390 Buy Now
Expressed In: Baculovirus Protein Species: Mouse

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for Recombinant JAK2 proteinContents

10 µg of Recombinant JAK2 protein in 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant JAK2 proteinApplication Notes

Recombinant JAK2 is suitable for kinase assays and Western blot. The molecular weight of the protein is 78.512 kDa. The activity of the protein is 24 pmol/µg min. ATP-KM: 2.1 μM Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), Substrate: TRK-C-derived Peptide (R11-VYSTDYYRLFNPS) at 50 µg/ml, Recombinant JAK2 at 4 μg/ml. Kinase activity may vary depending on the substrate and reaction conditions used.

for Recombinant JAK2 proteinProtein Details

Recombinant mouse JAK2 protein was produced using baculovirus infected Sf9 cells. The protein is a C-terminal fragment containing amino acids P717-G1132, accession number NP_004963.1 fused to a GST-HIS6-Thrombin cleavage site. Purified by GST-affinity chromatography.

 
Recombinant JAK2 protein, active analyzed by Coomassie.

Recombinant JAK2 protein Coomassie Stain
Coomassie stained gel of 2 µg of JAK2 protein.

JAK2 Kinase Activity Assay

JAK2 activity assay.
Recombinant JAK2 activity measured using a kinase assay.

for Recombinant JAK2 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant JAK2 protein
 

Data Thumbnails

Recombinant JAK2 protein, active analyzed by Coomassie.

Coomassie stain gel of Recombinant JAK2 protein, active.
(Click image to enlarge and see details.)

JAK2 Kinase Activity Assay

JAK2 Kinase Activity Assay.
(Click image to enlarge and see details.)