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Recombinant Proteins & Enzymes

Recombinant JAK3 protein

Aliases: Janus Kinase 3, JAK3-, Leukocyte Janus Kinase, L-JAK
Catalog No: 31182 Format: 10 µg $395 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant JAK3 proteinContents

10 µg of Recombinant JAK3 protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant JAK3 proteinApplication Notes

Recombinant JAK3 is suitable for kinase assays and Western blot. The molecular weight of the protein is ~68.379 kDa. The activity of the protein is ~ 8 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: R11-TRK-C-derived Peptide (R11-VYSTDYYRLFNPS) 2.5 µg / 50 µl, Recombinant JAK3 : 200 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.

for Recombinant JAK3 proteinProtein Details

Recombinant human JAK3 protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids I781-S1124, accession number NM_000215 and N-terminally fused to a GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.

Recombinant JAK3 protein, active analyzed by SDS-PAGE gel.

Recombinant JAK3 protein tested by SDS-PAGE gel.
0.5 µg of JAK3 protein was analyzed using SDS/PAGE followed by Western blot with either an anti-JAK3 or anti-GST antibody.

for Recombinant JAK3 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.


Technical Data Sheet

Recombinant JAK3 protein

Data Thumbnails

Recombinant JAK3 protein, active analyzed by SDS-PAGE gel.

SDS-PAGE gel of Recombinant JAK3 protein, active.
(Click image to enlarge and see details.)