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for Recombinant JNK3 protein, activeContents
10 µg of Recombinant JNK3 protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol. Protein is supplied at 0.126 µg/µl.
for Recombinant JNK3 protein, activeApplication Notes
Recombinant JNK3 is suitable for kinase assays and Western blot. The molecular weight of the protein is ~82.161 kDa. The activity of the protein is ~ 0.2 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: Rb-CTF 2 µg / 50 µl, Recombinant JNK3 : 200 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.
for Recombinant JNK3 protein, activeProtein Details
Recombinant human JNK3 protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids M1-Q422, accession number NM_002753 and N-terminally fused to GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.
Recombinant JNK3 protein tested by SDS-PAGE gel.
0.5 µg of JNK3 protein was analyzed using SDS/PAGE followed by Western blot with either an anti-JNK3 or anti-GST antibody.
for Recombinant JNK3 protein, activeGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
