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Recombinant LCK protein

Aliases: LCK Proto-Oncogene, Src Family Tyrosine Kinase, T Cell-Specific Protein-Tyrosine Kinase
Catalog No: 31184 Format: 10 µg $390 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant LCK proteinContents

10 µg of Recombinant LCK protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol. PPlease refer to product insert upon arrival for lot-specific concentration.

for Recombinant LCK proteinApplication Notes

Recombinant LCK is suitable for kinase assays and Western blot. The molecular weight of the protein is ~92.762 kDa. The activity of the protein is ~ 24 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: PolyEY 2 µg / 50 µl, Recombinant LCK : 200 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.

for Recombinant LCK proteinProtein Details

Recombinant human LCK protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids M1-P509, accession number NM_005356 and N-terminally fused to a GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.

 
Recombinant LCK protein, active analyzed by SDS-PAGE gel.

Recombinant LCK protein tested by SDS-PAGE gel.
0.5 µg of LCK protein was analyzed using SDS/PAGE followed by Western blot with either an anti-LCK or anti-GST antibody.

for Recombinant LCK proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant LCK protein
 

Data Thumbnails

Recombinant LCK protein, active analyzed by SDS-PAGE gel.

SDS-PAGE gel of Recombinant LCK protein, active.
(Click image to enlarge and see details.)