Your Cart
Browse
Filter
|
|
for Recombinant MAPKAPK5 protein, activeContents
10 µg of Recombinant MAPKAPK5 protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol. Protein is supplied at 0.113 µg/µl.
for Recombinant MAPKAPK5 protein, activeApplication Notes
Recombinant MAPKAPK5 is suitable for kinase assays and Western blot. The molecular weight of the protein is ~84.116 kDa. The activity of the protein is ~ 2 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: tetra (LRRWSLG), 3 µg / 50 µl, Recombinant MAPKAPK5 : 200 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.
for Recombinant MAPKAPK5 protein, activeProtein Details
Recombinant human MAPKAPK5 protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids M1-Q473, accession number NM_139078 and N-terminally fused to GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.
Recombinant MAPKAPK5 protein tested by SDS-PAGE gel.
0.5 µg of MAPKAPK5 protein was analyzed using SDS/PAGE followed by Western blot with either an anti-MAPKAPK5 or anti-GST antibody.
for Recombinant MAPKAPK5 protein, activeGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
