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Recombinant Proteins & Enzymes

Recombinant MAPKAPK5 protein

Aliases: Mitogen-Activated Protein Kinase-Activated Protein Kinase 5, MK5, MK-5, PRAK, MAPKAP-K5
Catalog No: 31186 Format: 10 µg $390 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant MAPKAPK5 proteinContents

10 µg of Recombinant MAPKAPK5 protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant MAPKAPK5 proteinApplication Notes

Recombinant MAPKAPK5 is suitable for kinase assays and Western blot. The molecular weight of the protein is ~84.116 kDa. The activity of the protein is ~ 2 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: tetra (LRRWSLG), 3 µg / 50 µl, Recombinant MAPKAPK5 : 200 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.

for Recombinant MAPKAPK5 proteinProtein Details

Recombinant human MAPKAPK5 protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids M1-Q473, accession number NM_139078 and N-terminally fused to a GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.

 
Recombinant MAPKAPK5 protein, active analyzed by SDS-PAGE gel.

Recombinant MAPKAPK5 protein tested by SDS-PAGE gel.
0.5 µg of MAPKAPK5 protein was analyzed using SDS/PAGE followed by Western blot with either an anti-MAPKAPK5 or anti-GST antibody.

for Recombinant MAPKAPK5 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant MAPKAPK5 protein
 

Data Thumbnails

Recombinant MAPKAPK5 protein, active analyzed by SDS-PAGE gel.

SDS-PAGE gel of Recombinant MAPKAPK5 protein, active.
(Click image to enlarge and see details.)