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for Recombinant PAK4 protein, activeContents
10 µg of Recombinant PAK4 protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol. Protein is supplied at 0.122 µg/µl.
for Recombinant PAK4 protein, activeApplication Notes
Recombinant PAK4 is suitable for kinase assays and Western blot. The molecular weight of the protein is ~93.968 kDa. The activity of the protein is ~ 8 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: tetra (LRRWSLG), 2.5 µg / 50 µl, Recombinant PAK4: 100 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.
for Recombinant PAK4 protein, activeProtein Details
Recombinant human PAK4 protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids M1-R591, accession number NM_005884 and N-terminally fused to GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.
Recombinant PAK4 protein tested by SDS-PAGE gel.
0.5 µg of PAK4 protein was analyzed using SDS/PAGE followed by Western blot with either an anti-PAK4 or anti-GST antibody.
for Recombinant PAK4 protein, activeGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
