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for Recombinant PDGFRb protein, activeContents
10 µg of Recombinant PDGFRβ protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol. Protein is supplied at 0.086 µg/µl.
for Recombinant PDGFRb protein, activeApplication Notes
Recombinant PDGFRβ is suitable for kinase assays and Western blot. The molecular weight of the protein is ~88.029 kDa. The activity of the protein is ~ 4 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: PolyAEKY 2.5 µg / 50 µl, Recombinant PDGFRβ: 10 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.
for Recombinant PDGFRb protein, activeProtein Details
Recombinant human PDGFRβ protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids R561-L1106, accession number NM_002609 and N-terminally fused to GST-Factor Xa cleavage site. Purified by GSH-agarose affinity purification.
Recombinant PDGFRβ protein tested by SDS-PAGE gel.
0.5 µg of PDGFRβ protein was analyzed using SDS/PAGE followed by Western blot with either an anti-PDGFRβ or anti-GST antibody.
for Recombinant PDGFRb protein, activeGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
