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Recombinant Proteins & Enzymes

Recombinant PDK1 protein

Aliases: Pyruvate Dehydrogenase Kinase 1, PDPK1, PDPK2, PDPK2P, PRO0461
Catalog No: 31192 Format: 10 µg $390 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant PDK1 proteinContents

10 µg of Recombinant PDK1 protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant PDK1 proteinApplication Notes

Recombinant PDK1 is suitable for kinase assays and Western blot. The molecular weight of the protein is ~81.331 kDa. The activity of the protein is ~ 6 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: tetra(LRRWSLG), 2.5 µg / 50 µl, Recombinant PDK1: 50 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.

for Recombinant PDK1 proteinProtein Details

Recombinant human PDK1 protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids M1-M460, accession number NM_002613 and N-terminally fused to GST-HIS6-Thrombin site. Purified by GSH-agarose affinity purification.

 
Recombinant PDK1 protein, active analyzed by SDS-PAGE gel.

Recombinant PDK1 protein tested by SDS-PAGE gel.
0.5 µg of PDK1 protein was analyzed using SDS/PAGE followed by Western blot with either an anti-PDK1 or anti-GST antibody.

for Recombinant PDK1 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant PDK1 protein
 

Data Thumbnails

Recombinant PDK1 protein, active analyzed by SDS-PAGE gel.

SDS-PAGE gel of Recombinant PDK1 protein, active.
(Click image to enlarge and see details.)