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for Recombinant SYK protein, activeContents
10 µg of Recombinant SYK protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol. Protein is supplied at 0.141 µg/µl.
for Recombinant SYK protein, activeApplication Notes
Recombinant SYK is suitable for kinase assays and Western blot. The molecular weight of the protein is ~104.408 kDa. The activity of the protein is ~ 12 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: PolyEY, 2 µg / 50 µl, Recombinant SYK: 200 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.
for Recombinant SYK protein, activeProtein Details
Recombinant human SYK protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids M1-N635, accession number NM_003177 and N-terminally fused to GST-HIS6-Thrombin site. Purified by GSH-agarose affinity purification.
for Recombinant SYK protein, activeGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
