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for Recombinant WEE1 protein, activeContents
10 µg of Recombinant WEE1 protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol. Protein is supplied at 0.15 µg/µl.
for Recombinant WEE1 protein, activeApplication Notes
Recombinant WEE1 is suitable for kinase assays and Western blot. The molecular weight of the protein is ~74.902 kDa. The activity of the protein is ~ 1 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: PolyAEKY, 1.5 µg / 50 µl, Recombinant WEE1: 400 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.
for Recombinant WEE1 protein, activeProtein Details
Recombinant human WEE1 protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids Q250-Y646, accession number X62048 and N-terminally fused to GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.
Recombinant WEE1 protein tested by SDS-PAGE gel.
0.5 µg of WEE1 protein was analyzed using SDS/PAGE followed by Western blot with either an anti-WEE1 or anti-GST antibody.
for Recombinant WEE1 protein, activeGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
