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Recombinant Proteins & Enzymes

Recombinant WEE1 protein

Aliases: WEE1A; WEE1hu
Catalog No: 31201 Format: 10 µg $390 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant WEE1 proteinContents

10 µg of Recombinant WEE1 protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant WEE1 proteinApplication Notes

Recombinant WEE1 is suitable for kinase assays and Western blot. The molecular weight of the protein is ~74.902 kDa. The activity of the protein is ~ 1 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: PolyAEKY, 1.5 µg / 50 µl, Recombinant WEE1: 400 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.

for Recombinant WEE1 proteinProtein Details

Recombinant human WEE1 protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids Q250-Y646, accession number X62048 and N-terminally fused to a GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.

 
Recombinant WEE1 protein, active analyzed by SDS-PAGE gel.

Recombinant WEE1 protein tested by SDS-PAGE gel.
0.5 µg of WEE1 protein was analyzed using SDS/PAGE followed by Western blot with either an anti-WEE1 or anti-GST antibody.

for Recombinant WEE1 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Technical Data Sheet

Recombinant WEE1 protein
 

Data Thumbnails

Recombinant WEE1 protein, active analyzed by SDS-PAGE gel.

SDS-PAGE gel of Recombinant WEE1 protein, active.
(Click image to enlarge and see details.)