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for Recombinant Yes protein, activeContents
10 µg of Recombinant Yes protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol. Protein is supplied at 0.116 µg/µl.
for Recombinant Yes protein, activeApplication Notes
Recombinant Yes is suitable for kinase assays and Western blot. The molecular weight of the protein is ~90.997 kDa. The activity of the protein is ~ 29 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: PolyEY, 1 µg / 50 µl, Recombinant Yes: 50 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.
for Recombinant Yes protein, activeProtein Details
Recombinant human Yes protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids M1-L543, accession number NM_005433 and N-terminally fused to GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.
Recombinant Yes protein tested by SDS-PAGE gel.
0.5 µg of Yes protein was analyzed using SDS/PAGE followed by Western blot with either an anti-Yes or anti-GST antibody.
for Recombinant Yes protein, activeGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
