Your Cart
Browse
Filter
|
|
for Recombinant GCN5 protein, activeContents
5 µg of Recombinant GCN5 protein, active. GCN5 is formulated in 20 mM Tris-HCl pH 7.3, 20% glycerol, 0.2 mM EDTA, 300 mM KCl, 1 mM DTT containing 0.5 mM PMSF.
for Recombinant GCN5 protein, activeApplication Notes
This protein has been tested for activity in the fluorescent HAT Assay Kit, Catalog No. 56100. 50 ng of GCN5 were assayed per well using 50 µM histone H3 peptide substrate, 50 µM Acetyl-CoA and incubated for 30 minutes at room temperature. The assay was read on a fluorescent plate reader (ex. 360-390 nm, em. 450-470 nm). The specific activity was 100 pmol/min/µg.
for Recombinant GCN5 protein, activeProtein Details
Recombinant FLAG-tagged GCN5 (amino acids 362-837) protein is expressed in E. coli and purified by affinity chromatography. Protein is supplied at 0.1 mg/ml. The Genbank Accession number for this GCN5 is U57316. The apparent MW is 55 kDa.
Recombinant GCN5 protein activity assay.
GCN5 activity assayed with the fluorescent HAT assay (Catalog No. 56100) using 50 ng of GCN5, 50 µM histone H3 peptide substrate (residues 5-23 of human H3), 50 µM Acetyl-CoA and 15 µM Anacardic Acid for inhibition. GCN5 was assayed alone to determine background and the resulting value was subtracted from this data.
for Recombinant GCN5 protein, activeGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
