Recombinant Histone H3 (C110A)

for Recombinant Histone H3 (C110A)Contents

100 µg supplied as lyophilized powder.

for Recombinant Histone H3 (C110A)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points.

for Recombinant Histone H3 (C110A)Application Notes

Recombinant histones are suitable for use as substrates for in vitro enzymatic reactions and chromatin assembly. Recombinant Histone H3 (C110A) is useful as a wild-type control for assays including applications that use recombinant methylated or acetylated histones.

for Recombinant Histone H3 (C110A)Protein Details

Recombinant Xenopus laevis Histone H3 (C110A) produced in E. coli and purified using FPLC. Protein concentration was determined by spectrophotometry. ≥98% purity by SDS-PAGE. It contains a substitution of cysteine for alanine at amino acid 110.

for Recombinant Histone H3 (C110A)Storage

Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant Histone H3 (C110A)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.