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Recombinant Proteins & Enzymes

Recombinant Histone H3 dimethyl Lys36 (H3K36me2)

Catalog No: 31218 Format: 50 µg $345 Buy Now

Expressed In:

E. coli

Protein Species:

Xenopus

for Recombinant Histone H3 dimethyl Lys36 (H3K36me2)Contents

50 µg supplied as lyophilized powder.

for Recombinant Histone H3 dimethyl Lys36 (H3K36me2)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points.

for Recombinant Histone H3 dimethyl Lys36 (H3K36me2)Application Notes

Recombinant histones are suitable for use as substrates for in vitro enzymatic reactions and chromatin assembly.

for Recombinant Histone H3 dimethyl Lys36 (H3K36me2)Protein Details

Recombinant methylated histones are specifically methylated via a chemical alkylation reaction that introduces an analog of methyl lysine. This specific chemical treatment enables the site and degree of methylation to be controlled precisely. Each methylation reaction is over 99% complete, as verified by high-resolution ESI-TOF mass spectrometry. All recombinant histones are also confirmed by SDS-PAGE and dot blot or immunoblot. The methyl-lysine analog closely mimics natural methylation, so these recombinant histones are perfect for any functional assay.

 
Recombinant Histone H3 dimethyl Lys36 analyzed by SDS-PAGE gel.

Recombinant Histone H3 dimethyl Lys36 tested by SDS-PAGE gel.
SDS-PAGE analysis of 1.5 µg Recombinant Histone H3 (C110A) (lane 2), Recombinant Histone H3 monomethyl Lys36 (lane 3), Recombinant Histone H3 dimethyl Lys36 (lane 4), and Recombinant Histone H3 trimethyl Lys36 (lane 5). Molecular weight marker is in lane 1.

for Recombinant Histone H3 dimethyl Lys36 (H3K36me2)Storage

Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant Histone H3 dimethyl Lys36 (H3K36me2)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.