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Recombinant Proteins & Enzymes

Recombinant UBA1 protein

Aliases: A1S9; A1ST; GXP1; UBE1; A1S9T; AMCX1; POC20; SMAX2; UBA1A; UBE1X; CFAP124
Catalog No: 31227 Format: 10 µg $395 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant UBA1 proteinContents

10 µg of recombinant protein supplied in 20 mM Tris-HCl, pH 7.3, 100 mM KCl, 0.2 mM EDTA and 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant UBA1 proteinBackground

UBA1, ubiquitin-like modifier activating enzyme 1 (also known as ubiquitin-activating enzyme E1 (UBE1), A1S9, A1S9T, GXP1 or SMAX2) is a protein involved in activating ubiquitin, which marks cellular proteins for degradation. Ubiquitination involves three classes of enzymes: E1s, ubiquitin-activating enzymes, E2s, ubiquitin-conjugating enzymes and E3s, ubiquitin-protein ligases. UBA1 is an E1 enzyme that activates ubiquitin by adenylating its C-terminal glycine residue with ATP to generate an ubiquitin-E1 thioester and free AMP. UBA1 may also function in DNA repair, as it is A1S9T and BN75 temperature sensitivity complementing. Mutations in UBA1 are associated with spinal muscular atrophy.

for Recombinant UBA1 proteinApplication Notes

Recombinant UBA1 protein is suitable for use in ubiquitination assays. A recommended starting point for the ubiquitination assay is 100 ng recombinant protein per reaction.

for Recombinant UBA1 proteinProtein Details

Recombinant UBA1 protein was expressed in E. coli as the full-length human protein (accession number CAA40296) with an N-terminal His-Tag. The molecular weight of the protein is ~120 kDa.

for Recombinant UBA1 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Technical Data Sheet

Recombinant UBA1 protein