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for Recombinant Ubiquitin G76AContents
10 µg of recombinant protein supplied at a concentration of 0.5 µg/µl in 20 mM Tris-HCl, pH 7.3, 100 mM KCl, 0.2 mM EDTA and 20% glycerol.
for Recombinant Ubiquitin G76ABackground
Ubiquitin is a small, highly conserved regulatory protein that exists in all eukaryotic cells. Ubiquitin is covalently attached to a range of target proteins in order to mark these proteins for proteasomal degradation. Ubiquitination involves three classes of enzymes: E1s, ubiquitin-activating enzymes, E2s, ubiquitin-conjugating enzymes and E3s, ubiquitin-protein ligases.
for Recombinant Ubiquitin G76AApplication Notes
Recombinant Ubiquitin G76A protein is suitable for use in ubiquitination assays as a negative control. A recommended starting point for the ubiquitination assay is 200-500 ng recombinant protein per reaction.
for Recombinant Ubiquitin G76AProtein Details
Recombinant Ubiquitin G76A protein was expressed in E. coli as amino acids 1-76 of human protein (accession number AAA72608) with an N-terminal poly-histidine tag. Recombinant ubiquitin G76A protein contains a substitution of a glycine residue to alanine at amino acid 76. The molecular weight of the protein is ~10.6 kDa.
for Recombinant Ubiquitin G76AGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
