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for Recombinant UBA3 protein, activeContents
10 µg of recombinant protein supplied at a concentration of 0.4 µg/µl in 20 mM Tris-HCl, pH 7.3, 100 mM KCl, 0.2 mM EDTA and 20% glycerol.
for Recombinant UBA3 protein, activeBackground
UBA3, ubiquitin-like modifier activating enzyme 3 (also known as UBE1C, ubiquitin-activating enzyme E1C, or hUBA3) is a subunit of the heterodimeric ubiquitin activating enzyme E1, which is specific for the Rub family of ubiquitin-like proteins (Ubls). UBA3 associates with NAE1 to form the UBA3-NAE1 E1 enzyme complex that activates the ubiquitin-like protein NEDD8. NEDD8 regulates cell division, signaling and embryogenesis.
for Recombinant UBA3 protein, activeApplication Notes
Recombinant UBA3 protein is suitable for use in neddylation assays. A recommended starting point for the neddylation assay is 100 ng recombinant protein per reaction.
for Recombinant UBA3 protein, activeProtein Details
Recombinant UBA3 protein was expressed in E. coli as the full-length human protein (accession number AAC27648) with an N-terminal poly-histidine tag. The molecular weight of the protein is ~51.4 kDa.
for Recombinant UBA3 protein, activeGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.


