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Recombinant Proteins & Enzymes

Recombinant NEDD8 protein, active

Catalog No: 31236 Format: 10 µg $335 Buy Now

Expressed In:

E. coli

Protein Species:

Human

for Recombinant NEDD8 protein, activeContents

10 µg of recombinant protein supplied at a concentration of 0.5 µg/µl in 20 mM Tris-HCl, pH 7.3, 100 mM KCl, 0.2 mM EDTA and 20% glycerol.

for Recombinant NEDD8 protein, activeBackground

NEDD8, neural precursor cell expressed, developmentally down-regulated 8 (also known as NEDD-8, FLJ43224, MGC104393, MGC125896, or MGC125897) is a ubiquitin-like protein (Ubl) that regulates cell division, signaling and embryogenesis. NEDD8 is activated by the heterodimeric ubiquitin activating enzyme E1 complex, UBA3-NAE1 E1, and the E2 enzyme UBE2M catalyzes the covalent attachment to target proteins in the presence of the appropriate E3 ligase. There are several proteases capable of removing NEDD8 from its protein conjugates.

for Recombinant NEDD8 protein, activeApplication Notes

Recombinant NEDD8 protein is suitable for use in neddylation assays. A recommended starting point for the neddylation assay is 200-500 ng recombinant protein per reaction.

for Recombinant NEDD8 protein, activeProtein Details

Recombinant NEDD8 protein was expressed in E. coli as amino acids 1-76 of human protein (accession number NP_006147) with an N-terminal poly-histidine tag. The molecular weight of the protein is ~10.5 kDa.

for Recombinant NEDD8 protein, activeGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Technical Data Sheet

Recombinant NEDD8 protein, active