Recombinant NEDD8 G76A protein

for Recombinant NEDD8 G76A proteinContents

10 µg of recombinant protein supplied at a concentration of 0.5 µg/µl in 20 mM Tris-HCl, pH 7.3, 100 mM KCl, 0.2 mM EDTA and 20% glycerol.

for Recombinant NEDD8 G76A proteinBackground

NEDD8, neural precursor cell expressed, developmentally down-regulated 8 (also known as NEDD-8, FLJ43224, MGC104393, MGC125896, or MGC125897) is a ubiquitin-like protein (Ubl) that regulates cell division, signaling and embryogenesis. NEDD8 is activated by the heterodimeric ubiquitin activating enzyme E1 complex, UBA3-NAE1 E1, and the E2 enzyme UBE2M catalyzes the covalent attachment to target proteins in the presence of the appropriate E3 ligase. There are several proteases capable of removing NEDD8 from its protein conjugates.

for Recombinant NEDD8 G76A proteinApplication Notes

Recombinant NEDD8 G76A protein is suitable for use in neddylation assays as a negative control. A recommended starting point for the neddylation assay is 200-500 ng recombinant protein per reaction.

for Recombinant NEDD8 G76A proteinProtein Details

Recombinant NEDD8 G76A protein was expressed in E. coli as amino acids 1-76 of human protein (accession number NP_006147) with an N-terminal poly-histidine tag. Recombinant NEDD8 G76A protein contains a substitution of a glycine residue to alanine at amino acid 76. The molecular weight of the protein is ~10.5 kDa.

for Recombinant NEDD8 G76A proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.