Contents:
10 µg of recombinant protein supplied at a concentration of 0.4 µg/µl in 20 mM Tris-HCl, pH 7.3, 125 mM NaCl, 0.2 mM EDTA and 20% glycerol.
Background:
PARP-1,
poly (ADP-ribose) polymerase family, member 1, (also known as
PARP1,
ADPRT,
ADP-ribosyltransferase NAD+, or
PPOL,
poly(ADP-ribose) polymerase) is an abundant and ubiquitous nuclear enzyme involved in cellular processes such as DNA damage repair, the regulation of chromatin structure and the control of gene expression. PARP-1 catalyzes the poly(ADP-ribosyl)ation of a variety of acceptor proteins involved in chromatin architecture and in DNA metabolism, including core histones, the linker histone H1 and a variety of transcription factors.
For additional information on PARP-1, please see the review article
PARP-1: An Abundant and Ubiquitous Protein with Roles in Many Cellular Processes in the Targets & Applications section or our website.
Protein Details:
Recombinant PARP-1 protein was expressed in Sf9 insect cells as the full-length human protein (accession number NP_001609) with an N-terminal FLAG tag. The molecular weight of the protein is ~115 kDa.
Protein Applications:
Recombinant PARP-1 protein is suitable for use in assays that modify histones and CTCF by poly ADP-ribosylation. A recommended starting point is 100 ng recombinant protein per reaction.
Storage:
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee:
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
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