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Recombinant Proteins & Enzymes

Recombinant DNA Topoisomerase I protein, active

Catalog No: 31239 Format: 10 µg $335 Buy Now

Expressed In:

Sf9 cells

Protein Species:

Drosophila melanogaster

for Recombinant DNA Topoisomerase I protein, activeContents

10 µg of recombinant protein supplied at a concentration of 0.15 µg/µl in 20 mM Tris-HCl, pH 7.3, 125 mM NaCl, 0.2 mM EDTA and 20% glycerol.

for Recombinant DNA Topoisomerase I protein, activeBackground

DNA topoisomerase I (TOP1) is an enzyme involved in the state of DNA during transcription. It catalyzes the breaking and rejoining of single stranded DNA, thus altering the topology of DNA.

for Recombinant DNA Topoisomerase I protein, activeApplication Notes

Recombinant DNA Topoisomerase I protein is suitable for use in assays that study DNA and chromatin topological changes. A recommended starting point is 5-20 ng recombinant protein per reaction.

for Recombinant DNA Topoisomerase I protein, activeProtein Details

Recombinant DNA Topoisomerase I protein was expressed in Sf9 insect cells as the full-length Drosophila melanogaster protein (accession number AAA28951) with an N-terminal FLAG tag. The molecular weight of the protein is ~135 kDa.

for Recombinant DNA Topoisomerase I protein, activeGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.