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for Recombinant VRK1 protein, activeContents
10 µg of recombinant protein supplied at a concentration of 0.5 µg/µl in 20 mM Tris-HCl, pH 7.3, 300 mM KCl, 0.2 mM EDTA and 20% glycerol.
for Recombinant VRK1 protein, activeBackground
VRK1, Vaccinia-related Kinase 1 belongs to a family of serine/threonine protein kinases. It phosphorylates histone, casein and transcription factors ATF2 and c-Jun. VRK-1 also phosphorylates Thr18 of p53/TP53 to reduce p53 ubiquitination. VRK1 is highly expressed in actively dividing cells, such as fetal liver, testis and thymus.
for Recombinant VRK1 protein, activeApplication Notes
Recombinant VRK1 protein is suitable for use in kinase assays. We recommended to start with a range of protein concentrations (e.g. 5 ng, 50 ng, and 500 ng) to determine the optimal amount needed for each assay.
for Recombinant VRK1 protein, activeProtein Details
Recombinant VRK1 protein was expressed in E. coli as the full-length human protein (accession number NP_003375) with an N-terminal FLAG tag. The molecular weight of the protein is ~48 kDa.
for Recombinant VRK1 protein, activeGuarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
