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for Recombinant APEX protein, activeContents
10 µg of recombinant protein supplied at a concentration of 0.2 µg/µl in 20 mM Tris-HCl, pH 7.3, 300 mM KCl, 0.2 mM EDTA and 20% glycerol.
for Recombinant APEX protein, activeBackground
APEX, APE/Ref-1 apurinic/apyrimidinic endonuclease (also known as APE, Ref1, AP endonuclease I or APEX nuclease) is a DNA repair enzyme that removes the blocking group from the 3’-termini of the DNA strand breaks generated by ionizing radiation. The apurinic/apyrimidinic (AP) sites can prevent normal DNA replication.
for Recombinant APEX protein, activeApplication Notes
Recombinant APEX protein is suitable for use in DNA repair assays. We recommended to start with a range of protein concentrations (e.g. 5 ng, 50 ng, and 500 ng) to determine the optimal amount needed for each assay.
for Recombinant APEX protein, activeProtein Details
Recombinant APEX protein was expressed in E. coli as the full-length human protein (accession number AAB22977) with an N-terminal FLAG tag. The molecular weight of the protein is ~38 kDa.
for Recombinant APEX protein, activeGuarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
