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for Recombinant Histone H2AContents
50 µg supplied as lyophilized powder.
for Recombinant Histone H2ABackground
Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points.
for Recombinant Histone H2AApplication Notes
Recombinant histones are suitable for use as substrates for in vitro enzymatic reactions and chromatin assembly. Each lot has been evaluated by SDS-PAGE and dot blot analysis.
for Recombinant Histone H2AProtein Details
Recombinant Xenopus laevis Histone H2A produced in E. coli and purified using FPLC. Protein concentration was determined by spectrophotometry. ≥98% purity by SDS-PAGE. Resuspend protein in suitable buffer for your assay.
Recombinant Histone H2A analyzed by SDS-PAGE gel.
5 µg, 2 µg and 1 µg respectively of Recombinant Histone H2B, Catalog No. 31252 (lanes 1-3), Recombinant Histone H2A, Catalog No. 31251 (lanes 4-6) and Recombinant Histone H3, Catalog No. 31207 (lanes 7-9).
for Recombinant Histone H2AStorage
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Recombinant Histone H2AGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
