Recombinant Histone H3 biotinylated

for Recombinant Histone H3 biotinylatedContents

25 µg supplied as lyophilized powder.

for Recombinant Histone H3 biotinylatedBackground

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points.

for Recombinant Histone H3 biotinylatedApplication Notes

Recombinant histones are suitable for use as substrates for in vitro enzymatic reactions and chromatin assembly.

for Recombinant Histone H3 biotinylatedProtein Details

Recombinant human Histone H3.2 produced in E. coli and purified using HPLC. The Recombinant H3 protein was linked to biotin at the N-terminus via a carbon linker. Protein concentration was determined by spectrophotometry and molar extinction coefficient. Protein was determined to have ≥ 98% purity by SDS-PAGE. The molecular weight is 15,565 Daltons.

for Recombinant Histone H3 biotinylatedStorage

Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant Histone H3 biotinylatedGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.