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Recombinant Proteins & Enzymes

Recombinant Histone H3.2 biotinylated (Human)

Catalog No: 31271 Format: 25 µg $395 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant Histone H3.2 biotinylated (Human)Contents

Recombinant Histone H3.2 biotinylated (Human) is supplied as lyophilized powder. Recombinant histones can be resuspended in water or any suitable buffer. We recommend a starting concentration of 1 mg/ml. To fully solubilize the histone we suggest resuspension in the buffer of choice at room temperature for 20-30 minutes with occasional pipetting. Addition of salt or Tris to the resuspension buffer may enhance histone solubility.

for Recombinant Histone H3.2 biotinylated (Human)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points.

for Recombinant Histone H3.2 biotinylated (Human)Application Notes

Recombinant histones are suitable for use as positive controls in the analysis of histone post-translational modifications, as substrates for histone modification enzymes, or to generate chromatin in vitro.

for Recombinant Histone H3.2 biotinylated (Human)Protein Details

Truncated recombinant Histone H3.2 biotinylated (Human) is produced in E. coli and purified using FPLC. The purified protein is subsequently ligated to a peptide containing the histone tail with a N-terminal biotin via a native peptide bond. The full-length protein is then repurified prior to lyophilization. Protein concentration was determined using the molar extinction coefficient for Histone H3 and absorbance at 280nm. Protein was determined to have ≥ 98% purity by SDS-PAGE. The molecular weight is 15,565 Daltons. To see a representative mass spectrum, please visit our website at www.activemotif.com and view the product details page.

 
Recombinant Histone H3 biotinylated analyzed by SDS-PAGE gel.

Recombinant Histone H3.2 biotinylated (Human) tested by SDS-PAGE gel.
SDS-PAGE analysis of 1.5 µg Recombinant Histone H3 biotinylated (lane 1) and 1 µg Recombinant Histone H3 biotinylated (lane 2). Molecular weight marker is in lane 4. Lane 3 is empty.

for Recombinant Histone H3.2 biotinylated (Human)Storage

Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant Histone H3.2 biotinylated (Human)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant Histone H3.2 biotinylated (Human)
 

Data Thumbnails

Recombinant Histone H3 biotinylated analyzed by SDS-PAGE gel.

SDS-PAGE gel of Recombinant Histone H3 biotinylated.
(Click image to enlarge and see details.)