Recombinant Histone H3 phospho Thr3 (H3T3ph)

for Recombinant Histone H3 phospho Thr3 (H3T3ph)Contents

25 µg supplied as lyophilized powder.

for Recombinant Histone H3 phospho Thr3 (H3T3ph)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points.

for Recombinant Histone H3 phospho Thr3 (H3T3ph)Application Notes

Recombinant histones are suitable for use as substrates for in vitro enzymatic reactions and chromatin assembly.

for Recombinant Histone H3 phospho Thr3 (H3T3ph)Protein Details

Recombinant human Histone H3.2 produced in E. coli and purified using FPLC. The purified protein was subsequently ligated to a phospho threonine 3 peptide via a native peptide bond and repurified prior to lyophilization. The recombinant histone is >98% pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrophotometry. Resuspend protein in suitable buffer for your assay.

for Recombinant Histone H3 phospho Thr3 (H3T3ph)Storage

Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant Histone H3 phospho Thr3 (H3T3ph)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.