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Recombinant Proteins & Enzymes

Recombinant Histone H3.3 biotinylated (Human)

Catalog No: 31297 Format: 100 µg $100 Buy Now
Catalog No: 31897 Format: 1 mg $500 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant Histone H3.3 biotinylated (Human)Contents

Full length recombinant Histone H3.3 biotinylated (Human) is supplied in 25 mM Tris pH 7.5, 150 mM NaCl, 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant Histone H3.3 biotinylated (Human)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points.

Histone H3.1 and Histone H3.3 are the two main Histone H3 variants found in plants and animals. They are known to be important for gene regulation. Histone H3.1 and H3.3 have been shown to demonstrate unique genomic localization patterns thought to be associated with their specific functions in regulation of gene activity. Specifically, Histone H3.1 localization is found to coincide with genomic regions containing chromatin repressive marks (H3K9me3, H3K27me3 and DNA methylation), whereas Histone H3.3 primarily colocalizes with marks associated with gene activation (H3K4me3, H2BK120ub1, and RNA pol II occupancy). Deposition of the Histone H3.1 variant into the nucleosome correlates with the canonical DNA synthesis-dependent deposition pathway, whereas Histone H3.3 primarily serves as the replacement Histone H3 variant outside of S-phase, such as during gene transcription. Aberrant localization of these variants is also known to correlate with certain cancers.

for Recombinant Histone H3.3 biotinylated (Human)Application Notes

Recombinant Histone H3.3 biotinylated (Human) is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

for Recombinant Histone H3.3 biotinylated (Human)Protein Details

Recombinant Histone H3.3 biotinylated (Human) corresponds to the native histone sequence of mammalian histone H3 variant H3.3 and does not contain any amino acid substitutions or residue analogs. Histone H3.3 is known to be important for gene regulation. Recombinant Histone H3.3 biotinylated (Human) consists of the full-length sequence of human histone H3.3 variant (accession number NM_005324) and contains a C-terminal Biotin tag. Recombinant Histone H3.3 biotinylated (Human) was generated in E. coli cells and has an observed molecular weight of 18 kDa. The recombinant protein is >75% pure by SDS-PAGE.

 
Recombinant Histone H3.3 - biotinylated, protein gel.

Recombinant Histone H3.3 biotinylated (Human) protein gel.
Recombinant Histone H3.3 - biotinylated was run on a 12% SDS-PAGE gel and stained with Coomassie Blue.

for Recombinant Histone H3.3 biotinylated (Human)Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant Histone H3.3 biotinylated (Human)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant Histone H3.3 biotinylated (Human)
 

Data Thumbnails

Recombinant Histone H3.3 - biotinylated, protein gel.

Recombinant Histone H3.3 - biotinylated, protein gel stained with Coomassie Blue.
(Click image to enlarge and see details).