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Recombinant Proteins & Enzymes

Recombinant Set9 H297A protein

Catalog No: 31320 Format: 10 µg $345 Buy Now

Expressed In:

E. coli

Protein Species:

Human

for Recombinant Set9 H297A proteinContents

10 µg recombinant Set9 H297A protein supplied at a concentration of 2 µg/µl in 20 mM Tris-HCl, pH 7.3, 100 mM KCl, 0.2 mM EDTA and 20% glycerol.

for Recombinant Set9 H297A proteinApplication Notes

Recombinant Set9 H297A is suitable for use in methyltransferase assays. A recommended starting point for the methyltransferase assay is 50-200 ng of recombinant protein per reaction. The molecular weight of the protein is ~53 kDa. This is a version of the protein with a catalytic site mutant, so it is designed for use as a negative control.

for Recombinant Set9 H297A proteinProtein Details

Set9 is a histone methyltransferase that is specific to lysine 4 of histone H3 (H3K4). Methylation of H3K4 is associated with transcriptional activation. Recombinant Set9 H297A was expressed as the full-length (accession number NP_085151.1) human protein with an N-terminal poly-histidine tag. The recombinant Set9 H297A protein contains a substitution of a conserved histidine residue within the SET domain to alanine at amino acid 297. Set9 H297A is catalytically inactive.

for Recombinant Set9 H297A proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.