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Recombinant Proteins & Enzymes

Recombinant PRMT1 E143Q protein

Catalog No: 31326 Format: 10 µg $345 Buy Now

Expressed In:

E. coli

Protein Species:

Human

for Recombinant PRMT1 E143Q proteinContents

10 µg recombinant PRMT1 E143Q protein supplied at a concentration of 2 µg/µl in 20 mM Tris-HCl, pH 7.3, 100 mM KCl, 0.2 mM EDTA and 20% glycerol.

for Recombinant PRMT1 E143Q proteinApplication Notes

Recombinant PRMT1 E143Q is suitable for use in methyltransferase assays. A recommended starting point for the methyltransferase assay is 50-200 ng of recombinant protein per reaction. The molecular weight of the protein is ~47 kDa. This is a version of the protein with a catalytic site mutant, so it is designed for use as a negative control.

for Recombinant PRMT1 E143Q proteinProtein Details

PRMT1 (protein arginine N-methyltransferase 1) is a type 1 PRMT, capable of monomethylating and asymmmetrically dimethylating arginine residues. PRMT1 specifically methylates arginine 3 on histone H4 (H4R3). Recombinant PRMT1 E143Q was expressed as the full-length (accession number NP_001527.3) human protein with an N-terminal poly-histidine tag. The recombinant PRMT1 E143Q protein contains a substitution of glutamic acid to glutamine at amino acid 143. PRMT1 E143Q is catalytically inactive.

for Recombinant PRMT1 E143Q proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.