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Recombinant Proteins & Enzymes

Recombinant G9a H904K protein

Catalog No: 31328 Format: 10 µg $345 Buy Now

Expressed In:

E. coli

Protein Species:

Human

for Recombinant G9a H904K proteinContents

10 µg recombinant G9a H904K protein supplied at a concentration of 2 µg/µl in 20 mM Tris-HCl, pH 7.3, 100 mM KCl, 0.2 mM EDTA and 20% glycerol.

for Recombinant G9a H904K proteinApplication Notes

Recombinant G9a H904K is suitable for use in methyltransferase assays. A recommended starting point for the methyltransferase assay is 50-200 ng of recombinant protein per reaction. The molecular weight of the protein is ~81 kDa. This is a version of the protein with a catalytic site mutant, so it is designed for use as a negative control.

for Recombinant G9a H904K proteinProtein Details

G9a is a histone methyltransferase that is specific to lysine 9 of histone H3 (H3K9). Methylation of H3K9 is a signal of transcriptional repression. Recombinant G9a H904K was expressed as amino acids 530-1210 of human protein (accession number NP_006700.3) with an N-terminal poly-histidine tag. The recombinant G9a H904K protein contains a substitution of histidine for lysine at amino acid 904. G9a H904K is catalytically inactive.

for Recombinant G9a H904K proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.