Recombinant TFIIA α/β protein

for Recombinant TFIIA alpha/beta proteinContents

10 µg recombinant TFIIA protein supplied at a concentration of 0.4 µg/µl in 20 mM Tris-HCl (pH7.3), 300 mM KCl, 0.2 mM EDTA and 20% glycerol.

for Recombinant TFIIA alpha/beta proteinBackground

TFIIA (also known as TF2A1, GTF2A1, general transcription factor IIA subunit 1). TFIIA is a component of RNA polymerase II transcription complex. TFIIA associates with TATA-binding subunit (TBP) to regulate transcriptional activity.TFIIA binds directly to TBP and stabilizes its binding to DNA, perhaps through direct contacts of its own with the DNA. TFIIA acts as an anti-repressor, stabilizing TFIID binding by blocking repressors of transcription that inhibit binding of other transcription factors or that remove TBP from the DNA. Activation of transcription may be dependent on this TFIIA function. TFIIA is comprised of multiple subunits, TFIIA1 and TFIIA2. TFIIA1 has two subunits, TFIIA alpha and TFIIA beta, which are processed from the same polypeptide precursor.

for Recombinant TFIIA alpha/beta proteinApplication Notes

Recombinant TFIIA protein is suitable for use in in vitro transcription assays. We recommend to start with a range of protein concentrations (e.g. 5 ng, 50 ng, and 500 ng) to determine the optimal amount needed for each in vitro transcription assay.

for Recombinant TFIIA alpha/beta proteinProtein Details

Recombinant TFIIA protein was expressed in E. coli as the full-length human protein (accession number CAA54442) with an N-terminal FLAG tag. The molecular weight of the protein is ~55 kDa.

for Recombinant TFIIA alpha/beta proteinStorage

Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant TFIIA alpha/beta proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.