Recombinant EZH2 protein complex, active

for Recombinant EZH2 complex protein, activeContents

25 µg recombinant EZH2 protein complex (also contains EED and SUZ12 proteins) supplied at a concentration of 0.44 µg/µl in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide and 10% glycerol.

for Recombinant EZH2 complex protein, activeApplication Notes

Recombinant EZH2 complex is suitable for use in histone methylatransferase assays. A recommended starting point for the HMT assay is 20-100 ng of recombinant protein per reaction.

for Recombinant EZH2 complex protein, activeProtein Details

EZH2 is a histone methyltransferase specific for H3 lysine 27, a mark of repressed regions of chromatin. EZH2 is only active as part of a multi-subunit protein complex, PRC2 (Polycomb Repressive Complex 2). Recombinant human EZH2 (accession number NP_004447, amino acids 2-751 with an N-terminal His tag, molecular weight 86 kDa) was co-expressed in Sf9 cells along with human EED (accession number NP_003788, amino acids 2-441 with an N-terminal FLAG tag, molecular weight 51 kDa) and human SUZ12 (accession number NP_056170, amino acids 2-739 with an N-terminal His tag, molecular weight 87 kDa).

for Recombinant EZH2 complex protein, activeStorage

Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant EZH2 complex protein, activeGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.