|
|
for Recombinant SIRT1 protein, activeContents
10 µg recombinant SIRT1 protein supplied at a concentration of 0.2 µg/µl in 20 mM Tris-HCl (pH7.3), 300 mM KCl, 0.2 mM EDTA and 20% glycerol.
for Recombinant SIRT1 protein, activeApplication Notes
Recombinant SIRT1 is suitable for use in histone deacetylase assays. A recommended starting point for the HDAC assay is 25-100 ng of recombinant protein per reaction.
for Recombinant SIRT1 protein, activeProtein Details
SIRT1 is a histone and protein deacetylase, an NAD-dependant enzyme that removes acetyl groups from lysine acetylated proteins. SIRT1 has been shown to deacetylate histone H4 at lysine 16. Recombinant human SIRT1 was expressed in E. coli as the full length protein (accession number AAD40849) with an N-terminal FLAG tag. The molecular weight of the protein is ~120 kDa.
for Recombinant SIRT1 protein, activeGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.


