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for Recombinant TFIIE beta proteinContents
10 µg recombinant TFIIE β protein supplied at a concentration of 0.1 µg/µl in 20 mM Tris-HCl (pH7.3), 300 mM KCl, 0.2 mM EDTA and 20% glycerol.
for Recombinant TFIIE beta proteinBackground
TFIIE beta, transcription factor II E beta (also known as TFIIEB, T2E2, general transcription factor IIE polypeptide 2, or GTF2E2) is required for promoter clearance by RNA polymerase, along with TFIIH.
for Recombinant TFIIE beta proteinApplication Notes
Recombinant TFIIE β protein is suitable for use in in vitro transcription assays. We recommend to start with a range of protein concentrations (e.g. 5 ng, 50 ng, and 500 ng) to determine the optimal amount needed for each in vitro transcription assay.
for Recombinant TFIIE beta proteinProtein Details
Recombinant TFIIE β protein was expressed in E. coli as the full-length human protein (accession number NP_002086) with an N-terminal FLAG tag. The molecular weight of the protein is 35 kDa.
for Recombinant TFIIE beta proteinStorage
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Recombinant TFIIE beta proteinGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
