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Recombinant Proteins & Enzymes

Recombinant Carm1 / Prmt4 protein

Aliases: Mouse Carm1, Mouse Prmt4
Catalog No: 31347 Format: 20 µg $395 Buy Now
Catalog No: 31747 Format: 1 mg $3,500 Buy Now
Expressed In: Baculovirus Protein Species: Mouse

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for Recombinant Carm1 / Prmt4 proteinContents

Full length recombinant mouse Carm1 / Prmt4 protein supplied in 25 mM HEPES (pH 7.5), 300 mM NaCl, 5% glycerol and 0.2 mg/ml 3X FLAG peptide. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant Carm1 / Prmt4 proteinBackground

Carm1 / Prmt4 (Coactivator-associated arginine methyltransferase 1 or Carm1) is a protein arginine N-methyltransferase that catalyzes the mono- and asymmetric dimethylation of arginine residues in its substrate proteins. It serves as a coactivator of transcription and also plays a role in nuclear receptors. Carm1 methylates histone H3 at Arg2, Arg17 and Arg26; it also methylates a number of non-histone substrates involved in the regulation and mechanism of gene expression.

for Recombinant Carm1 / Prmt4 proteinApplication Notes

Recombinant Mouse Carm1 / Prmt4 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

HMT Assay Conditions: 30 ng/μl Histone H3.1 was incubated with 30 ng/μl recombinant Carm1 / Prmt4 protein in reaction buffer including 50 mM TrisCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 9 nCi/μl 3H-SAM for 2 hours at room temperature. Activity was detected by fluorography.

for Recombinant Carm1 / Prmt4 proteinProtein Details

Carm1 / Prmt4 is protein arginine methyltransferase specific for histone H3. Recombinant mouse Carm1 / Prmt4 was expressed in Sf9 cells (accession number NP_067506) with an N-terminal FLAG-Tag. The molecular weight of the protein is ~67 kDa.

 
Carm1 / Prmt4 Coomassie gel

Recombinant Carm1 / Prmt4 protein gel.
Carm1 / Prmt4 protein was run on an SDS-PAGE gel and stained with Coomassie blue.

Carm1 / Prmt4 activity assay

Carm1 / Prmt4 activity assay.
HMT assay measuring the activity of Recombinant Carm1 / Prmt4. Activity was detected by fluorography.

Carm1 / Prmt4 activity assay

Carm1 / Prmt4 activity assay.
Recombinant Carm1 / Prmt4 activity measured using a demethylation assay. MALDI-TOF was used for detection.

for Recombinant Carm1 / Prmt4 proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant Carm1 / Prmt4 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant Carm1 / Prmt4 protein
 

Data Thumbnails

Carm1 / Prmt4 Coomassie gel

Carm1 / Prmt4 Coomassie stained gel.
(Click image to enlarge and see details).

Carm1 / Prmt4 activity assay

Carm1 / Prmt4 activity assay.
(Click image to enlarge and see details).

Carm1 / Prmt4 activity assay

Carm1 / Prmt4 activity assay.
(Click image to enlarge and see details).