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Recombinant Proteins & Enzymes

Recombinant Carm1 E267Q protein

Aliases: Prmt4
Catalog No: 31348 Format: 10 µg $395 Buy Now
Expressed In: Baculovirus Protein Species: Mouse

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for Recombinant Carm1 E267Q proteinContents

10 µg recombinant Carm1 E267Q protein supplied in 20 mM Tris-HCl (pH7.3), 300 mM KCl, 0.2 mM EDTA and 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant Carm1 E267Q proteinApplication Notes

Recombinant Carm1 E267Q is suitable for use in histone methyltransferase assays. A recommended starting point for the methyltransferase assay is 20-100 ng of recombinant protein per reaction. This is a version of the protein with a catalytic site mutant, so it is designed for use as a negative control.

for Recombinant Carm1 E267Q proteinProtein Details

Carm1 (PRMT4) is protein arginine methyltransferase specific for histone H3. Recombinant mouse Carm1 was expressed in Sf9 cells (accession number Q9WVG6) with an N-terminal FLAG-Tag. The molecular weight of the protein is ~65 kDa. This protein was expressed with a catalytic domain mutation (E267Q).

for Recombinant Carm1 E267Q proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant Carm1 E267Q proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant Carm1 E267Q protein