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Recombinant Proteins & Enzymes

Recombinant CARM1 E267Q protein

Catalog No: 31348 Format: 10 µg $345 Buy Now

Expressed In:

Sf9 cells

Protein Species:

Mouse

for Recombinant CARM1 E267QContents

10 µg recombinant CARM1 E267Q protein supplied at a concentration of 0.5 µg/µl in 20 mM Tris-HCl (pH7.3), 300 mM KCl, 0.2 mM EDTA and 20% glycerol.

for Recombinant CARM1 E267QApplication Notes

Recombinant CARM1 E267Q is suitable for use in histone methyltransferase assays. A recommended starting point for the methyltransferase assay is 20-100 ng of recombinant protein per reaction. This is a version of the protein with a catalytic site mutant, so it is designed for use as a negative control.

for Recombinant CARM1 E267QProtein Details

CARM1 (PRMT4) is protein arginine methyltransferase specific for histone H3. Recombinant mouse CARM1 was expressed in Sf9 cells (accession number Q9WVG6) with an N-terminal FLAG tag. The molecular weight of the protein is ~65 kDa. This protein was expressed with a catalytic domain mutation (E267Q).

for Recombinant CARM1 E267QStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant CARM1 E267QGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.