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Recombinant HDAC5 protein, catalytic domain, active
| Catalog No: 31351 | Format: 10 µg | $345 | Buy Now |
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for Recombinant HDAC5, catalytic domain, activeContents
10 µg recombinant HDAC5, catalytic domain expressed in Sf9 cells supplied in a buffer of 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 imidazole, and 10% glycerol.
for Recombinant HDAC5, catalytic domain, activeApplication Notes
Recombinant HDAC5, catalytic domain is suitable for use in histone deacetylase (HDAC) assays. A good starting point is 20 to 50 ng of enzyme per assay.
for Recombinant HDAC5, catalytic domain, activeProtein Details
HDAC5 is a Class II histone deacetylase with broad specificity. The catalytic domain (amino acids 657-1123) of human HDAC5 (accession number NP_001015053.1), was expressed with a C-terminal His tag (MW= 51 kDa) in Sf9 cells using a baculovirus expression system.
HDAC5 activity assay.
Recombinant HDAC5 activity measured using a fluorescent HDAC assay.
HDAC5 protein gel.
HDAC5 (3 μg) run on an SDS-PAGE gel and stained with Coomassie blue.
for Recombinant HDAC5, catalytic domain, activeStorage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Recombinant HDAC5, catalytic domain, activeGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
