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for Recombinant MST1 protein, activeContents
5 µg recombinant Mst1 expressed in Sf9 cells supplied in a buffer of 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 3 mM DTT, 18 mM glutathione, and 10% glycerol.
for Recombinant MST1 protein, activeApplication Notes
Recombinant MST1 is suitable for use in kinase assays. A good starting point is 5 to 25 ng of enzyme per assay.
for Recombinant MST1 protein, activeProtein Details
MST1 (Mammalian STE20-like protein kinase 1, STK4, KRS2) is a kinase that phosphorylates histone H2B at serine 14 during apoptosis and in response to double-strand DNA breakage. Amino acids 2-488 of MST1 (accession number NP_006273.1) were expressed with an N-terminal GST-tag, (MW=82 kDa) in Sf9 cells using a baculovirus expression system.
MST1 activity assay.
Recombinant MST1 activity measured using a fluorescent kinase assay.
MST1 protein gel.
MST1 (6 μg) run on an SDS-PAGE gel and stained with Coomassie blue.
for Recombinant MST1 protein, activeStorage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Recombinant MST1 protein, activeGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
