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for Recombinant PRMT5 complex protein, activeContents
20 µg recombinant PRMT5 complex expressed in Sf9 cells supplied in a buffer of 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 ng/µl Flag peptide, 20% glycerol, and 3 mM DTT.
for Recombinant PRMT5 complex protein, activeApplication Notes
Recombinant PRMT5 complex is suitable for use in histone methyltransferase (HMT) assays. A good starting point is 10 to 100 ng of enzyme per assay.
for Recombinant PRMT5 complex protein, activeProtein Details
PRMT5 is an arginine methyltransferase that is responsible for the symmetric dimethylation of histone H2A Arg3, histone H3 Arg8 and histone H4 Arg3. N-terminally FLAG tagged PRMT5 (accession number NM_006109, MW=73 kDa) were co-expressed with N-terminally His tagged MEP50 (accession number NM_024102, MW=37 kDa) in Sf9 cells using a baculovirus expression system.
PRMT5 activity assay.
Recombinant PRMT5 activity measured using a luminescent methyltransferase assay.
PRMT5 protein gel.
PRMT5 complex (4.5 μg) run on an SDS-PAGE gel and stained with Coomassie blue.
for Recombinant PRMT5 complex protein, activeStorage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Recombinant PRMT5 complex protein, activeGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
