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Recombinant Proteins & Enzymes

Recombinant SUV420H1 protein

Aliases: CGI85; KMT5B; CGI-85
Catalog No: 31360 Format: 50 µg $395 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant SUV420H1 proteinContents

50 µg recombinant SUV420H1 supplied in a buffer of 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 3 mM DTT. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant SUV420H1 proteinApplication Notes

Recombinant SUV420H1 is suitable for use in histone methyltransferase (HMT) assays. Specific Activity: 0.0009 pmol/min/μg. This protein is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Assay conditions: Prepare a 50 μl reaction mix (50 mM TRIS pH 8.8, 5 mM MgCl2, 4 mM DTT, 0.05% Tween-20, 800 μM S-adenosylmethionine, and SUV420H1) and add to substrate-coated wells. Incubate for 4 hr. Add primary antibody, incubate 1 hr. Next, incubate 30 minutes with the HRP-labeled secondary antibody followed by chemiluminescent detection.

for Recombinant SUV420H1 proteinProtein Details

SUV420H1 is a histone methyltransferase specific for dimethylation of Histone H4 at lysine 20. Amino acids 2-387 of human SUV420H1 (accession number NM_017635) were expressed in E. coli with an N-terminal GST-Tag (MW = 70 kDa).

 
SUV420H1 Coomassie gel

SUV420H1 protein gel.
SUV420H1 run on a 4-20% SDS-PAGE gel and stained with Coomassie blue. Arrow indicates recombinant SUV420H1.

SUV420H1 activity assay

SUV420H1 activity assay.
Recombinant SUV420H1 activity measured using a fluorescent histone methyltransferase assay.

for Recombinant SUV420H1 proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant SUV420H1 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant SUV420H1 protein
 

Data Thumbnails

SUV420H1 Coomassie gel

SUV420H1 Coomassie stained gel.
(Click image to enlarge and see details).

SUV420H1 activity assay

SUV420H1 activity assay.
(Click image to enlarge and see details).