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for Recombinant UbcH6 protein, activeContents
50 µg recombinant UbcH6 expressed in E. coli supplied in a buffer of 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, 20% glycerol, and 3 mM DTT.
for Recombinant UbcH6 protein, activeApplication Notes
Recombinant UbcH6 is suitable for use in ubiquitin conjugating assays. A good starting point is 25 to 100 ng of enzyme per assay.
for Recombinant UbcH6 protein, activeProtein Details
UbcH6 is an E2 ubiquiquitin conjugating enzyme responsible for the monoubiquitination of histone H2B at lysine 120. Amino acids 2-193 of UBCH6 (accession number NP_003332.1) were expressed in E. coli with an N-terminal His tag (MW= 22 kDa).
UbcH6 activity assay.
Recombinant UbcH6 activity measured using a luminescent ubiquitin conjugating assay.
UbcH6 protein gel.
UbcH6 (10 μg) run on an SDS-PAGE gel and stained with Coomassie blue. Arrow indicates recombinant UbcH6.
for Recombinant UbcH6 protein, activeStorage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Recombinant UbcH6 protein, activeGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
