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Recombinant Proteins & Enzymes

Recombinant RXR-LBD protein

Aliases: RAR, NR1B1
Catalog No: 31365 Format: 100 µg $395 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant RXR-LBD proteinContents

100 µg of recombinant RXR-LBD protein expressed in E. coli and is provided in 50 mM Tris pH 8.0, 150 mM NaCl, 1mM DTT and 50% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant RXR-LBD proteinBackground

The Retinoid X receptor (RXR) ligand binding domain (LBD) is a promiscuous heterodimerization domain for binding of RXR to many nuclear receptors, including thyroid hormone receptors (TRs), retinoic acid receptors (RARs), peroxisome proliferator-activated receptor, orphan nuclear receptors and oxysterol receptors. Binding of RXR-LBD to nuclear receptors is mediated by the identity box, a 40-amino acid subregion within the ligand binding domain. The LBD is responsible for mediating transcription through receptor homo- and heterodimerization. The specific function of the RXR-containing complexes in regulating transcription depends on the associated ligand. For example, hormone binding to the LBD of the RXR results a conformational change that affects the C-terminal transactivation helix H12 involved in transcriptional activation. RXR-LBD ligand binding triggers dissociation of corepressor and the recruitment of coactivators, such as members of the Src family kinases, NCOA2/GRIP1, and RAC3/AIB1 which, in turn, promote transcription of downstream targets.

for Recombinant RXR-LBD proteinApplication Notes

Recombinant RXR-LBD is suitable for in vitro transcription, ligand binding and protein-protein interaction assays. 20 ng is sufficient for in vitro transcription assays and 100 ng is sufficient for ligand binding and protein-protein interaction studies.

for Recombinant RXR-LBD proteinProtein Details

RXR-α is a nuclear receptor that forms heterodimers with retinoic acid receptors (RAR). The ligand binding domain (LBD) corresponding to amino acids 223 - 463 of RXR-α (accession number 1609194A) was expressed in E. coli with an apparent molecular weight of 27 kDa. An epitope tag present during expression was removed proteolytically after purification.

 
RXR-LBD Coomassie gel.

RXR-LBD protein gel.
RXR-LBD run on an SDS-PAGE gel and stained with Coomassie blue.

for Recombinant RXR-LBD proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant RXR-LBD proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Technical Data Sheet

Recombinant RXR-LBD protein
 

Data Thumbnails

RXR-LBD Coomassie gel.

RXR-LBD Coomassie stained gel.
(Click image to enlarge and see details).