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Recombinant Proteins & Enzymes

Recombinant NCOR2 (SMRT)-DAD protein

Aliases: NCOR2; TRAC; CTG26; SMRTE; TRAC1; N-CoR2; TNRC14; TRAC-1; SMAP270; SMRTE-tau
Catalog No: 31366 Format: 100 µg $405 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant NCOR2 (SMRT)-DAD proteinContents

100 µg of recombinant NCOR2 (SMRT)-DAD protein expressed in E. coli at a concentration of 0.49 mg/ml in 50 mM Tris pH 8.0, 150 mM NaCl, 1mM DTT and 50% glycerol.

for Recombinant NCOR2 (SMRT)-DAD proteinBackground

NCOR2 (SMRT)-DAD, is a transcriptional corepressor that associates with a subset of nuclear receptors including RAR, PR, GR and TR in the absence of ligand. It is part of a large co-repressor protein complex that includes SIN3A/B and histone deacetylases HDAC1 and HDAC2. The deacetylase activating domain (DAD) functions to bind and activate histone deacetylase 3 (HDAC3) and is also required for N-CoR interaction with HDAC3 and its corepressor activity. SMRT and N-CoR mediated transcriptional corepression plays a significant role in development and metabolism and aberrant corepressor activity is associated with the pathogenesis of myeloid leukemias.

for Recombinant NCOR2 (SMRT)-DAD proteinApplication Notes

Recombinant NCOR2 (SMRT)-DAD is suitable for use in histone deacetylase (HDAC) assays as it is sufficient for activation of certain HDACs, including HDAC3. Use equimolar with the HDAC used in the assay.

for Recombinant NCOR2 (SMRT)-DAD proteinProtein Details

NCOR2 (SMRT)-DAD is a transcriptional corepressor that associates with a subset of nuclear receptors including RAR, PR, GR and TR in the absence of ligand. The deacetylase activating domain (DAD) corresponding to amino acids 420 - 480 of NCOR2 (SMRT)-DAD (accession number Q9Y618.2) was expressed in E. coli and has an apparent molecular weight of 9 kDa. An epitope tag present during expression was removed proteolytically after purification.

 
NCOR2 (SMRT)-DAD Coomassie gel.

NCOR2 (SMRT) -DAD protein gel.
NCOR2 (SMRT) -DAD run on an SDS-PAGE gel and stained with Coomassie blue.

for Recombinant NCOR2 (SMRT)-DAD proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant NCOR2 (SMRT)-DAD proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Technical Data Sheet

Recombinant NCOR2 (SMRT)-DAD protein
 

Data Thumbnails

NCOR2 (SMRT)-DAD Coomassie gel.

NCOR2 (SMRT)-DAD Coomassie stained gel.
(Click image to enlarge and see details).