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Recombinant Proteins & Enzymes

Recombinant BRPF1 (627-746) protein

Aliases: BR140, Peregrin
Catalog No: 31375 Format: 100 µg $395 Buy Now
Catalog No: 31875 Format: 1 mg $2,600 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant BRPF1 (627-746) proteinContents

Recombinant BRPF1 (627-746) protein expressed in E. coli and provided in 25 mM Tris pH 7.4, 150 mM NaCl and 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant BRPF1 (627-746) proteinBackground

Bromodomain and PHD finger containing (BRPF1) protein is a component of the MOZ/MORF complex which has histone H3 acetyltransferase activity. The bromodomain of BRPF1 recognizes acetylated histone lysine residues and function as a ‘reader’ of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. BRPF1 is known to positively regulate the transcription of RUNX1 and RUNX2.

for Recombinant BRPF1 (627-746) proteinApplication Notes

Recombinant BRPF1 (627-746) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

for Recombinant BRPF1 (627-746) proteinProtein Details

The peptide corresponding to amino acids 627 - 746 that contains the bromodomain sequences of BRPF1 (accession number NM_001003694.1) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with an observed molecular weight of 20.3 kDa. The recombinant protein is >95% pure by SDS-PAGE.

 
BRPF1 (627-746) Coomassie gel

Recombinant BRPF1 (627-746) protein gel.
BRPF1 (627-746) protein was run on an SDS-PAGE gel and stained with Coomassie Blue.

BRPF1 (627-746) HTRF activity assay

Recombinant BRPF1 (627-746) HTRF activity assay.
3.3 µM histone peptide H4K5/8/12/16(4Ac) was incubated with BRPF1 (627-746) protein in reaction buffer including 50mM HEPES-NaOH pH 7.0, 0.1% BSA overnight at room temperature. Anti-Flag antibody was used to detect reaction products.

for Recombinant BRPF1 (627-746) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant BRPF1 (627-746) proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant BRPF1 (627-746) protein
 

Data Thumbnails

BRPF1 (627-746) Coomassie gel

BRPF1 (627-746) Coomassie stained gel.
(Click image to enlarge and see details).

BRPF1 (627-746) HTRF activity assay

BRPF1 (627-746) HTRF activity assay.
(Click image to enlarge and see details).